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体外筛选剪切DNA的DNA酶

In Vitro Selection of DNA-cleaving DNAzyme
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摘要 为得到能够剪切单链DNA序列的人工DNA酶,通过体外筛选的方法,得到了一类DNA酶F-9.F-9的结构由两侧与底物结合区域和中间的酶活性中心组成;F-9在一定的Cu2+、Mn2+离子浓度下发挥催化作用,可以与底物结合形成二级结构.F-9可以剪切DNA序列,底物识别区域ACTG为保守序列.同时,对DNA酶的剪切位点作了进一步研究,证明F-9是一个多位点剪切的DNA酶. In order to obtain single-standed^-DNA-cleaving artificial DNA enzyme, we carried out in vitro selection and got the DNAzyme F-9 that consists of a catalytic domain flanked by two single-strand binging arms. F-9 DNA enzyme can cleave DNA in a certain concentration of copper and manganese metal ions. F-9 can cleave DNA sequence containing ACTG. Study of the splice site of DNA substrate found that F-9 undergoes cleavage at several closely-grouped sites. Fig 6, Tab 1, Ref 19
作者 张华帆 王明齐 陈浩东 董娟 杜凤 周丽 唐卓
机构地区 中国科学院成都生物研究所 四川大学化学学院
出处 《应用与环境生物学报》 CAS CSCD 北大核心 2013年第2期360-364,共5页 Chinese Journal of Applied and Environmental Biology
基金 中国科学院基金项目(KSCX2-EW-J-22)资助~~
关键词 体外筛选 DNA酶 催化活性 剪切DNA in vitro selection DNAzyme catalytic activity DNA-cleaving
分类号 Q55 [生物学—生物化学] Q78 [生物学—分子生物学]
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参考文献19

  • 1Breaker RR, Joyce GF. A DNA enzyme that cleaves RNA [J]. Chem Biol, 1994, 1 (4): 223-229.
  • 2Breaker RR, Joyce GF. A DNA enzyme with Mg^2+ dependent RNA phosphoesterase activity [J]. Chem Biol, 1995, 2 (10): 655-660.
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二级参考文献20

  • 1Breaker RR.Making catalytic DNAs[J],2000.
  • 2Breaker RR.Catalytic DNA:in training and seeking employment[J],1999(5).
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  • 6Cairns MJ;Hopkins TM;Witherington C.Target site selection for an RNA-cleaving catalytic DNA[J],1999(5).
  • 7Sriram B;Banerjea AC.In vitro-selected RNA cleaving DNA enzymes from a combinatorial library are potent inhibitors of HIV-1 gene expression[J],2000(Part 3).
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  • 10Liu C;Cheng R;Sun LQ.Supression of platelet-type 12-lipoxygenase activity in human erythroleukemia cells by an RNA-cleaving DNAzyme[J],2001(4).

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应用与环境生物学报
2013年 第2期

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