摘要
目的:离体条件下葡萄糖孵育SD大鼠乳鼠心脏成纤维细胞,观察罗格列酮干预后,对乳鼠心脏成纤维细胞表达Ⅰ型前胶原的影响,从而探讨罗格列酮在糖尿病心肌病心肌纤维化中的作用机制。方法:提取、培养新生SD大鼠心脏成纤维细胞,设对照组(葡萄糖5.5mmol.L-1);低糖5.5mmol.L-1+罗格列酮组;高糖30mmol.L-1组;高糖30mmol.L-1+罗格列酮组。应用Real time PCR法测定心脏成纤维细胞Ⅰ型前胶原-mRNA表达;Western blot检测Ⅰ型前胶原蛋白质的表达。结果:罗格列酮对高糖孵育下心脏成纤维细胞Ⅰ型前胶原-mRNA/protein表达的影响:与对照组相比,高糖刺激下Ⅰ型前胶原-mRNA/protein表达均增加(P<0.01)。与高糖30mmol.L-1组相比,高糖30mmol.L-1+罗格列酮组Ⅰ型前胶原-mRNA/protein表达降低(P<0.05)。结论:罗格列酮抑制了高血糖环境内心脏成纤维细胞胶原的合成,可能在抑制心肌纤维化的过程中发挥了作用。
OBJECTIVE To observe the impact of rosiglitazone on collagen I synthesis expression in neonatal rat cardiac fi- broblast cell, and to explore the mechanism of action of rosiglitazone in myocardial fibrosis of diabetes cardio-myopathy. METHODS SD neonatal rat cardiac fibroblasts were incubated in vitro and after the intervention of rosiglitazone. Neonatal SD rat cardiac fibroblasts were extracted and cultured.. （1）control group （normal glucose 5. 5 mmol.L-1 ）, （2）high glucose（30 mmol. L-1 ）, （3） low glucose （5. 5 mmol L- 1 ） and rosiglitazone, （4） high glucose （30 mmol L- 1 ） and rosiglitazone. Real time PCR was used to detect the expression of precollagen I mRNA of neonatal rat cardiac fibroblasts and Western blot was used to detect protein of precollagen I. RESULTS The influence of precollagen I -mRNA/protein under high glucose （30 rnmol. L- t ） by ros- iglitazone： compared to the control group, the expression of precollagen I-mRNA/protein in high glucose 30 mmol.L-1 was increased. There was statistically significant differences（P〈0. 01）. Moreover, precollagen I-mRNA/protein expression in high glucose 30 mmol-L-1 ＋ rosiglitazone group was significantly lower than high glucose 30 mmol. L-1 group. The difference was significant （P〈0. 05）. CONCLUSION Rosiglitazone inhibits the expression of collagen secretion rat cardiac fibroblasts in the environment of hyperglycemia, which maybe plays a role in the process of inhibition of myocardial fibrosis.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2013年第9期703-706,共4页
Chinese Journal of Hospital Pharmacy
