健脑益智胶囊对颅脑损伤大鼠血清NSE含量的影响

Effect of Jiannao Yizhi Capsules on Blood NSE in Modle Rats with Traumatic Brain Injury

目的:研究健脑益智胶囊对颅脑损伤后大鼠血清NSE含量的影响。方法:将SD大鼠按照随机原则分成4组,即:A组(假手术组),B组(模型对照组),C组(吡拉西坦治疗组),D组(健脑益智胶囊治疗组);每组再按照标本采集时间随机分为4个亚组(即A1、A2、A3、A4;B1、B2、B3、B4;C1、C2、C3、C4;D1、D2、D3、D4),每组10只。采用自由落体方法造模后,C组给予吡拉西坦治疗,D组给予健脑益智胶囊治疗,造模后第1d、3d、5d、7d、10d观察大鼠一般状况(精神状态、进食量、行为活动度)和血清NSE的含量变化并进行分析。结果:颅脑损伤后大鼠血清NSE水平较假手术组明显升高(P<0.01),受伤后大鼠血清NSE水平随时间的推移逐渐降低;C和D组NSE下降水平较明显;第7d,D组血清NSE水平较B组和C组明显降低,有显著差异(b、c:P<0.05),接近A组,两组无明显差异(a:P>0.05);第10d,C组和D组血清NSE水平较B组明显降低,两组间有明显差异(f、g:P<0.05),接近A组,两组间无明显差异(d、e:P>0.05)。结论:健脑益智胶囊在受伤后能促进TBI后大鼠血清NSE含量的降低,改善TBI后症状,可能与其能减轻脑组织及神经细胞的继发性损伤有关。

Objective：This study was to discuss the influence of Jiannao Yizhi Capsule on serum level of NSE of rats with traumatic brain injury.Methods：SD rats were randomly divided into four groups：A group（sham operation group）,B group（model control group）,C group（piracetam group）,D group（Jiannao Yizhi Capsule group）.Each group was randomly divided into four sub-groups according to the sample collecting times（A1、A2、A3、A4;B1、B2、B3、B4;C1、C2、C3、C4;D1、D2、D3、D4）with 10 in each group.After modeling with the method of free falling,C group was given piracetam and D group was given Jiannao Yizhi Capsule.On the 1st,3rd,5th,7the day,the common conditions of rats were observed（mental state,food-intake and behavior activity）and NSE changes in serum were analyzed.Results：The NSE level in serum was obviously increased in sham operation after traumatic brain injury（P0.01）,and the NSE level was gradually decreased as the time passing.The decrease of NSE in C and D groups was more obvious.On 7th day,the NSE level in serum in D group was significantly decreased than that in B and C groups and there was obvious difference（b,c：P0.05）;and the NES level in D group was similar to that in A group and there was no difference（a：P0.05）.On 10th day,the NSE level in serum in C and D groups were obviously decreased than that in B group and there was significant difference（f,g：P0.05）;and the NSE level in C and D groups was similar to that in A group without differences（d,e：P0.05）.Conclusion：After traumatic brain injury,Jiannao Yizhi Capsules can improve the decrease of NSE in serum and relieve the symptoms after TBI.It may be related to relieving brain tissue injury and secondary injury of nerve cells.