重组人乳铁蛋白致敏性的初步研究

Preliminary assessment of recombinant human lactoferrin for allergenic potential

目的初步评价牛乳腺生物反应器表达的重组人乳铁蛋白(rhLf)的致敏性。方法通过生物信息学分析和消化稳定性试验了解rhLf致敏的可能性。结果生物信息学分析显示rhLf与已知致敏原乳转铁蛋白和卵转铁蛋白有序列同源性,与Asp f 2、Ole e 10和Ole e 9存在结构相似性。消化稳定性试验显示rhLf在胃液中易被消化成小片段,在肠液中不易被消化。结论 rhLf具有一定的潜在致敏可能性。

Objective To evaluate the allergenic potential of recombinant human lactoferrin （rhLf） expressed by genetically modified cow in milk. Methods Bioinformatics searches and digestibility assay were conducted to investigate the allergenic potential of rhLf. Results Bioinformatics searches showed that there was high-level similarity between rhLf and some known allergens, such as lactotransferrin and ovotransferrin in amino acid sequence, and Asp f 2, Ole e 10 and Ole e 9 in structure. Digestibility assay showed that rhLf broke down into small fragment rapidly during gastric fluid digestion, while it was resistant to intestinal fluid digestion. Conclusion RhLf has allergenic potential.