超高效液相色谱法同时测定包衣糖果中5种铝色淀

Simultaneous determination of five aluminum lake dyes in coated candy by ultra performance liquid chromatography

目的建立包衣糖果中柠檬黄、日落黄、胭脂红、诱惑红和亮蓝5种铝色淀的超高效液相色谱测定方法。方法用NaOH溶液浸泡提取包衣糖果中的色淀,以乙腈-乙酸铵(10 mmol/L)为流动相,梯度洗脱,多波长检测定量。结果 5种目标化合物在0.25～50 mg/L范围内呈良好的线性,相关系数R2>0.999。该方法中5种目标化合物的定量限(LOQ)均为1.0 mg/kg。在1、5、25 mg/kg三个添加浓度水平下,5种目标化合物的加标回收率为79.5%～116.4%,RSD为0.92%～6.48%。结论该方法简单、快速、准确,适于包衣糖果中5种铝色淀的同时检测。

Objective To develop a method for simultaneous determination of tartrazine A1 lake, sunset yellow A1 lake, poneeau 4R A1 lake, allura red A1 lake and brilliant blue A1 lake in coated candy by ultra performance liquid chromatography. Methods Target gradient elution with anetonitrile an detector. Results Good linearities correlation coefficients R2 〉 0. 999. analytes in coated candy were extracted by sodium hydroxide solution, separated by d 10 mmol/L acetic ammonium as mobile phase, and detected by photodiode array were achieved for five aluminum lake dyes in the range of 0. 25-0. 50 mg/L, with The quantification limits of the method were 1.0 mg/kg. Mean recoveries of five target analytes （spiked at three concentration levels） ranged from 79. 5% to 116.4% , with relative standard deviations between 0.92% and 6. 48%. Conclusion This simple, rapid and accurate method could be applied in simultaneous detection of five aluminum lake dyes in coated candy.