逍遥散对慢性束缚应激下脑区NMDA受体NR2A和NR2B表达的影响

Influence of Xiaoyao powder on expression of NR2A and NR2B in brain NMDA receptor by chronic restraint stress

目的观察海马及杏仁核(BLA)N-甲基-D-天冬氨酸(NMDA)受体NR2A和NR2B蛋白在束缚应激状态下的表达变化及逍遥散对其的调节作用。方法使用捆绑的方法制作慢性束缚应激动物模型,并用逍遥散进行干预,分别于7日后和21日后检测模型大鼠海马CA1区、CA3区、齿状回(DG)及BLA中NMDA受体NR2A和NR2B的蛋白表达情况。结果 NR2A和NR2B在海马的CA1、CA3、DG区和BLA中均有不同程度表达。逍遥散21日组海马CA1、CA3、DG区和BLA中NR2A和NR2B表达均上调(P<0.05或P<0.01);海马CA3和DG区中尤其明显(P<0.01)。结论逍遥散对慢性束缚应激引起的海马和BLA神经传递改变有明显的调节作用,可影响突触可塑性,适应机体的功能,主要靶点在海马的CA3和DG区。

Objective To observe the change of protein expressions of NR2A and NR2B in N-methyl-D-aspartic （NMDA） receptor in hippocampus and basolateral lateral amygdaloid （BLA） on restraint stress as well as the regulation action of Xiaoyao powder. Methods With tie-up method to establish animal model of chronic restraint stress, Xiaoyao powder was applied to intervene. The expression status of proteins in CA1, CA3, dentate gyrus （DG） of hippocampus, and NR2A, NR2B in NMDA receptor of BLA were tested after 7 and 21 days respectively. Results There was expression of NR2A and NR2B to varying degrees in CA1, CA3, DG of hippocampus and BLA. The expression of NR2A and NR2B in the Xiaoyao powder group on Day 21 was all increased in CA1, CA3, DG and BLA （P 〈 0.05, P 〈 0.01）, which was especially obvidus in CA3 and DG of hippocampus （P 〈 0.01）. Conclusion The Xiaoyao powder has obvious regulation action on chronic restraint stress-induced neurotransmission change in hippocampus and BLA, which could make an influence on plasticity of synapsis to satisfy body function, and the main target is CA3 and DG in hippocampus.