吡嗪酰胺为内标的高效毛细管电泳法定量血浆中盐酸川芎嗪

Establishment of a Quantitative Method for Tetramethylpyrazine Hydrochloride in Rat Plasma Using Pyramide as Internal Standard

目的建立以吡嗪酰胺为内标,高效毛细管电泳定量血浆中盐酸川芎嗪的分析方法。方法用未涂层熔融石英毛细管(75μm×60 cm,有效长度为50 cm)分离,背景电解质为100 mmol·L-1SDS-30 mmol·L-1硼砂缓冲液(40∶60),分离电压为20 kV,检测波长为295 nm,毛细管温度为20℃,进样条件3.448 kPa。结果分析方法的线性范围是1.12～286.72 mg·L-1(r=0.999 6),日内精密度RSD值小于4.21%,日间精密度RSD值小于4.74%,平均回收率是91.22%～97.48%,检测限是0.16 mg·L-1。结论该方法快速、准确,适合生物样品中盐酸川芎嗪的定量分析。

OBJECTIVE To develop an HPCE method of tetramethylpyrazine hydrochloride in rat plasma using pyramide as the internal standard. METHODS The separation was performed on a fused-silica uncoated capillary with an inner diameter （ID） of 75 μm, an effective length of 50 cm and a total length of 60 cm. The optimum HPCE conditions were as follows ：100 mmol · L^-1 SDS-30 mmol · L^-1 sodium tetraborate solution （40： 60） was used as the background electrolyt, the samples were injected at the anodic end at 0. 5 psi for 6 s, separation voltage was 20 kV at positive power, capilla^7 temperature was 20 ℃, and detection wavelength was 295 nm. RESULTS The linear range was 1.12 -286. 72 mg · L^-1 （ r = 0. 999 6） , and the inter-day and intra-day precisions expressed as RSDs were less than 4. 21% and 4. 74% , respectively. The average recoveries were 91.22% - 97.48% , and the limit of detection was 0. 16 mg · L ^-1. CONCLUSION This method is rapid and accurate to quantify tetramethylpyrazine hydrochloride in rat plasma.