蛹虫草多糖对小鼠脾脏淋巴细胞体外增殖的影响

Effect of Cordyceps militaris polysaccharide on proliferation of mouse spleen lymphocytes in vitro

为了筛选并验证蛹虫草中具有免疫活性的多糖,试验采用MTT法,在小鼠脾脏淋巴细胞培养体系中加入刀豆蛋白A(ConA)及终浓度为1,0.5,0.25 mg/L蛹虫草多糖培养48 h,观察其对小鼠脾脏淋巴细胞体外增殖的影响。结果表明:蛹虫草多糖可显著促进体外培养的小鼠脾脏淋巴细胞增殖。说明蛹虫草多糖具有免疫增强活性。

To verify immune activity of Cordyceps militaris polysaecharide （CMP） , the MTF method was used in the test, and then the mouse spleen lymphocytes were incubated in RPMI- 1640 tissue culture medium supplemented with ConA （final concentration of 5 rag/L）and CMP （ concentrations of 1, 0.5, 0.25 mg/L ） for 48 h. The in vitro proliferation of mouse spleen lymphocytes was observed. The results showed that CMP could significantly promote the proliferation of mouse spleen lymphocytes within O. 25 to 1 mg/L. h indicates that CMP has immune - enhancing activity.