摘要
目的:研究类叶升麻苷(acteoside)对冈田酸(OA)诱导的SK-N-SH神经细胞损伤的影响。方法:以20 nmol.L-1的冈田酸处理SK-N-SH神经细胞,建立AD样细胞模型,并以5,10,20 mg.L-1的类叶升麻苷拮抗其作用。通过倒置显微镜观察细胞形态,采用MTT比色法检测细胞存活率,酶标法试剂盒测定LDH渗漏率,应用Western blot技术检测神经细胞磷酸化tau蛋白的表达情况。结果:类叶升麻苷可明显改善SK-N-SH的细胞形态,提高细胞的存活率,降低细胞LDH的漏出率,下调磷酸化tau蛋白在p-Ser 199/202,p-Ser 404位点的表达,上调非磷酸化tau蛋白在Ser 202,Ser 404位点的表达。结论:类叶升麻苷对冈田酸诱导的SK-N-SH神经细胞损伤具有明显保护作用。
Objective： To investigate the effect of acteoside on SK-N-SH nerve cell injury induced by okadaic acid （OA）. Method： SK-N-SH nerve cells were processed with 20 nmol· L-l OA to establish the Alzheimer＇s disease （AD） cellular model, and 5, 10, 20 mg · L-1 acteoside was used to antagonize against its effect. Cell morphology was observed under inverted microscope. The cell survival rate was detected with MTT, and the LDH release rate was measured by enzyme label kit. Western blot was applied to de- termine the expression of phosphorylation tau proteins in nerve cells. Result： The acteoside could significantly improve SK-N-SH cell morphology, enhance the cell survival rate, decrease the cell LDH release rate and the expression of phosphorylated tau proteins at p-Ser 199/202 and p-Ser 404 sites, up-regulated the expression of at non-phosphorylated tau proteins at Ser 202 site and Ser 404 sites. Conclusion： Acteoside has significant protective effect on nerve cell injury induced by OA.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第9期1323-1326,共4页
China Journal of Chinese Materia Medica
基金
新疆维吾尔自治区高技术研究发展计划(201110104)
