夏枯草花粉离体萌发观察

Observations of in vitro pollen germination of Prunella vulgaris

选用唇形科植物夏枯草花粉为材料,通过在离体条件下花粉的萌发来探讨夏枯草花粉萌发的最适条件。以10%H3BO4,30%Ca(NO3)2,20%MgSO4,10%KNO3配制成无机盐溶液为基本培养基,统计不同相对分子质量的PEG,不同浓度的蔗糖,不同pH和不同温度条件以及不同采集时间下花粉萌发率的情况。夏枯草花粉萌发的最适液体培养条件是:10%H3BO4+30%Ca(NO3)2+20%MgSO4+10%KNO3+25%PEG-4000,pH在6.5左右,刚刚开花时采集的花粉在此条件下花粉萌发率最高。

To determine the optimal condition of pollen germination. The pollen of Prunella vulgaris was cultured in vitro. Pollen germination rates were recorded using 10% H3BO4,30% Ca（NO3 ）2, 20% MgSO4 and 10% KNO3 as the basic mineral medi- um with PEG of different molecular weight, sucrose of various density and multiple pH value. The rates were also measured under dif- ferent cultivation temperature and pollen acquisition time. The optimal condition of pollen germination is 10% H3 BO4, 30% Ca（NO3 ） 2, 20% MgSO4, 10% KNO3, and 25% PEG-4000 as the medium, with pH about 6.5 and pollen acquired at the beginning of blossom.