安息香在大鼠体内的药代动力学研究

Study on Pharmacokinetics of Benzoinum in Rats

目的建立安息香主要成分苯甲酸在大鼠体内的RP-HPLC测定方法,研究安息香在大鼠体内的药代动力学规律。方法 Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇-水-磷酸(50∶50∶0.05,V/V);流速为1.0ml.min-1;检测波长为228 nm;柱温:25℃。结果主要药代动力学参数为:雌性Cmax=(0.055 8±0.050 8)mg.ml-1,tmax=(1±0)h,t1/2=(0.168±0.084 5)h,AUC(0～∞)=(0.076 3±0.076 7)h.mg-1.ml-1;雄性Cmax=(0.077 4±0.0369)mg.ml-1,tmax=(1±0)h,t1/2=(0.533±0.417 4)h,AUC(0～∞)=(0.115 4±0.058 1)h.mg-1.ml-1。结论所建立的RP-HPLC方法操作简便、准确灵敏、重复性好,可用于安息香在大鼠体内药代动力学研究。

Objective To establish a RP-HPLC method for the determination of Benzoic acid and to find the regulation of pharmacokinetics of Benzoinum in rat plasma. Methods The Agilent TC-C18 chromatographic column(4.6 mm ×250 mm,5 μm) was used.Methanol-water-orthophosphoric acid(50:50:0.05,V/V) as the mobile phase at a flow rate of 1.0 ml·min-1and column temperature was at 25 ℃,the detection wavelength was at 228 nm. Results The main pharmacokinetic parameters were as follows:Cmax=0.0558 ± 0.0508 mg·ml-1,tmax=(1±0)h,t1/2=(0.168±0.0845) h,AUC(0~∞)=(0.0763±0.0767) h·mg-1·ml-1 for female;Cmax=(0.0774 ± 0.0369) mg-1·ml-1,tmax=1±0 h,t1/2 = 0.533 ± 0.4174 h,AUC(0~∞)=0.1154 ±0.0581 h·mg-1·ml-1 for male. Conclusion The method was simple,sensitive,accurate and reproducible,which was suitable for pharmacokinetic studies of Benzoinum in rat plasma.