摘要
目的考察罗汉果甜苷在PC12细胞中对外源性氧化损伤的保护作用。方法采用MTT法考察0.01～100μg/ml罗汉果甜苷对正常PC12细胞活力的影响,并在H2O2刺激的PC12细胞中考察0.01～10μg/ml的罗汉果甜苷对细胞活力的影响,通过对细胞活力的考察选择罗汉果甜苷合适的实验剂量,测定罗汉果甜苷0.1及1μg/ml对H2O2刺激的PC12细胞中过氧化氢酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力的影响以及对丙二醛(MDA)含量的影响。结果罗汉果甜苷在1～10μg/ml浓度能提高正常PC12细胞活力,在0.1～10μg/ml浓度下能显著提高H2O2刺激的PC12细胞活力(P<0.01)。0.1及1μg/ml两个剂量的罗汉果甜苷均能显著提高H2O2刺激的PC12细胞中SOD活力(P<0.001)和GSH-Px活力(P<0.001),并减少MDA生成(P<0.05)。结论罗汉果甜苷能缓解氧化应激导致的PC12细胞活力下降,并能增强PC12细胞的抗氧化能力。
Objective To investigate the protective effect of mogroside on hydrogen peroxide(H2O2) induced oxidative stress in PC12 cell. Methods MTT method was employed to determine the viability in mogroside(0.01~100 μg/mL) treated PC12 cell and mogroside(0.01~10 μg/mL) plus H2O2 treated PC12 cell.Thereafter,activity of SOD,GSH-Px,as well as MDA concentration were determined at optimal dosage of mogroside selected based on MTT experiment. Results PC12 cell viability was increased by mogroside at 1~10 μg/mL,and the viability of H2O2 treated PC12 cell was increased by mogroside at 0.1~10 μg/mL.Furthermore,mogroside at 0.1 and 1 μg/mL significantly enhanced SOD activity(P<0.001) and GSH-Px activity(P<0.001),decreased MDA concentration(P<0.05). Conclusion Mogroside alleviated loss of cell viability in H2O2 treated PC12 cell,the anti-oxidative capacity could enhanced by pre-treat with mogroside in PC12 cell line.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2013年第4期818-820,共3页
Lishizhen Medicine and Materia Medica Research
基金
中国博士后科学基金(No.20110490870)
广西自然科学基金创新研究团队项目(No.2011GXNSFF018006)
