玉郎伞多糖对Aβ_(25-35)诱导PC12细胞损伤的保护作用研究

Protective Effects of YLS Polysaccharides on Aβ_(25-35) Induced PC12 Cells Injury

目的:研究玉郎伞多糖(YLSPS)对Aβ25-35诱导PC12细胞损伤的保护作用。方法:采用Aβ25-35诱导PC12细胞损伤模型。试验分为6组,即空白对照(无血清DMEM低糖培养基)、模型(10μmol/LAβ25-35溶液)、石杉碱甲(1μmol/L石杉碱甲溶液)与YLSPS高、中、低浓度(1.0、0.1、0.01μg/mlYLSPS溶液,10μmol/LAβ25-35溶液)组。检测细胞培养液与细胞匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)、一氧化氮(NO)、一氧化氮合酶(NOS)、丙二醛(MDA)水平。结果:细胞培养液和细胞匀浆中,与模型组比较,YLSPS高、中、低浓度组NO、MDA含量显著减少、NOS活性显著减弱,SOD、GSH-Px活性显著增强,GSH含量显著增加(P<0.01或P<0.05)。结论:YLSPS对Aβ25-35诱导PC12细胞损伤具有较好的抗氧化保护作用。

OBJECTIVE： To study the antioxidative effect of YLS polysaccharides （YLSPS） on Aβ25-35 induced PC12 cells injury. METHODS： The Aβ25-35 induced PC12 cell injury model. The experiment was divided into 6 groups, blank control （without serum DMEM culture medium with low glucose）, model （10 μmol/L Aβ25-35 solution）, huperzine A（1 μmol/L of huperzine a solution） and YLSPS high,medium, low concentration （1, 0.1, 0.01 μ g/ml YLSPS solution, 10 μmol/L Aβ25-35 solution） groups. Detection of cell culture supernatant and cell superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, glutathione （GSH）, nitric oxide （NO）, nitric oxide synthase （NOS）, malondialdehyde （MDA） levels. RESULTS： In the cell culture medium and the cell cytoplasm, compared with model group, MDA and NO content decreased significantly, NOS activity decreased significantly, SOD and GSH-Px activity were enhanced significantly, GSH content increased significantly（P〈0.01 or P〈0.05） in YLSPS high , mdium, low concentration groups. CONCLUSIONS：YLSPS efficiently protect PC12 cells from oxidative damage induced by Aβ25-35.