黑面神水提物抗皮肤Ⅰ型超敏反应的研究

Experimental Study on Antagonizing TypeⅠHypersensitivity Effect of Water-extract of Breynia fruticosa

目的:研究黑面神水提物抗皮肤I型超敏反应的药理作用。方法:内源性组胺和外源性组胺实验均分为空白对照(等容蒸馏水)、扑尔敏(4mg/kg)与黑面神水提物高、中、低剂量(18、9、4.5g/kg)组,灌胃给药,每天1次,连续5d。末次给药1h后尾静脉注射0.025%低分子右旋糖酐-40(1.25mg/kg)诱发小鼠阵发性皮肤瘙痒模型,记录小鼠30min内皮肤瘙痒发作次数与时间;末次给药1h后于小鼠腹部正中脱毛皮肤处皮内注射0.1%磷酸组胺(0.1ml/10g),观察毛细血管通透性。结果:与空白对照组比较,黑面神水提物高、中、低剂量组小鼠30min内瘙痒发作次数显著减少,瘙痒持续时间显著缩短,毛细血管通透性显著降低(P<0.01或P<0.05)。结论:黑面神水提物可通过抑制组胺的释放发挥抗皮肤I型超敏反应作用。

OBJECTIVE： To investigate the pharmacological effect of water-extract of Breynia fruticosa antagonizing type Ⅰ hypersensitivity. METHODS： Endogenous and exogenous histamine experiments were divided into blank control（constant volume of distilled water）, chlorphenamine maleate （4 mg/kg）, water-extract of B. fruticosa high-dose, medium-dose and low-dose （18, 9, 4.5 g/kg） groups. They were given medicine intragastrically once a day for consecutive 5 days. Paroxysmal skin pruritus mice mod- el was induced by injecting with 0.025 % low molecular dextran （1.25 mg/kg） via tail vein 1 h after last medication. The frequency and time of paroxysmal skin pruritus attack were recorded within 30 min. 1 h after last medication, 0.1% histamine phosphate （0.1 ml/10 g） was injected intracutaneously on wound surface of abdominal middle region in mice. The permeability of capillary vessel was observed. RESULTS： Compare with blank control group, the frequency and duration of paroxysmal skin pruritus attack were decreased significantly, while the permeability of capillary vessel was decreased apparently in water-extract of B. fruticosa high-dose, medium-dose and low-dose groups （P〈0.01 or P〈0.05）. CONCLUSIONS：The water-extract of B. fruticosa could restrain type Ⅰ hypersensitivity by suppressing release of histamine.