丹参对听功能损伤模型豚鼠的保护作用研究

Study of the Protective Effect of Salvia miltiorrhiza on Hearing Function Damage Model of Guinea Pig

目的:研究丹参对听功能损伤模型豚鼠的保护作用。方法:连续5d腹腔注射顺铂2mg/kg以复制豚鼠听功能损伤模型。实验分为3组,即对照(等容生理盐水,5d)、模型、丹参(8ml/kg,7d)组,腹腔注射给药,每天1次。测定豚鼠听性脑干反应(ABR)阈值与Ⅰ波潜伏期变化;透射电镜观察内耳形态变化。结果:与对照组比较,模型组豚鼠ABR阈值显著升高、Ⅰ波潜伏期显著延长(P<0.01);与模型组比较,丹参组豚鼠ABR阈值显著降低、Ⅰ波潜伏期显著缩短(P<0.01)。丹参组内耳毛细胞变性坏死程度较模型组明显减轻。结论:丹参对顺铂导致的内耳损伤有一定的保护作用。

OBJECTIVE： To study the effects of Salvia miltiorrhiza on heating function in cisplatin injury model guinea pigs. METHODS： The hearing function injury model was induced by intraperitoneal injection of cisplatin 2 mg/kg for consecutive 5 days. The model rats were divided into 3 groups, i.e. control（constant volume of normal saline, 5 d）, model, S. miltiorrhiza（8 mY kg, 7 d）groups. They were given medicines intraperitoneally once a day. The changes of auditory brainstem response （ABR） threshold and latency of wave 1 were determined, and the morphological changes of inner ear were observed by transmission electron microscope. RESULTS： Compared with control group, ABR threshold in model group increased significantly, and latency of wave I prolonged significantly （P〈0.01）;compared with model group, ABR threshold in S. miltiorrhiza group decreased significantly, and latency of wave I shortened significantly（P〈0.01）. The necrosis of bristle cells in inner ear in S. miltiorrhiza group was slighter than in model group. CONCLUSIONS ：S. miltiorrhiza shows protective effects against cisplatin-induced injury to the inner ear.