维药菟丝草的质量标准研究

Study on Quality Standard of Uygur Medicine Cuscuta chinensis

目的:建立维药菟丝草的质量标准。方法:采用薄层色谱(TLC)法对菟丝草进行定性鉴别;采用高效液相色谱法同时测定菟丝草中金丝桃苷和槲皮素的含量:色谱柱为Phenomenex-PackGemini-NX5UC18110A(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(18∶82,V/V),检测波长为360nm,柱温为30℃,流速为1ml/min。结果:TLC鉴别特征明显、分离度好。金丝桃苷和槲皮素的质量浓度分别在4.50～45.00、1.37～13.70μg/ml范围内与各自峰面积积分值呈良好线性关系(r分别为0.9997、0.9998);二者精密度、重复性、稳定性试验的RSD均<2%;平均加样回收率分别为102.11%和98.66%,RSD分别为0.47%和1.71%(n均为6)。结论:所建标准可用于菟丝草的质量控制。

OBJECTIVE： To establish the quality standard of Uygur medicine Cuscuta chinensis. METHODS：C. chinensis was identified by TLC. The contents of hyperin and quercetin were determined by HPLC. The determination was performed on Phe- nomenex-Pack Gemini-NX 5U C18 110A （250 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-0.1% phosphoric acid（ 18 ： 22, V/V） at the flow rate of 1 ml/min. The detection wavelength was set at 360 nm and the column temperature was 30 ℃. RESULTS： TLC spots were clear and well-separated. The linear range of hyperin and quercetin were 4.50-45.00 μg/ml （r= 0.999 7）and 1.37-13.70 μg/ml （r=0.999 8）, respectively. RSDs of precision test,reproducibility test and stability test were all lower than 2 %. The average recoveries were 102.11% （RSD=0.47%, n=6） and 98.66% （RSD= 1.71%, n= 6）. CONCLUSIONS ： Established standard can be used for the quality control of C. chinensis.