双波长HPLC法同时测定安乳散结丸中芍药苷与迷迭香酸的含量

Simultaneous Determination of Paeoniflorin and Rosmarinic Acid in Anru Sanjie Pill by Dual-wavelength HPLC

目的:建立同时测定安乳散结丸中芍药苷与迷迭香酸含量的方法。方法:采用双波长高效液相色谱法。色谱柱为AgilentEclipsepluseC18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸水(20∶80,V/V),检测波长为230nm(芍药苷)和330nm(迷迭香酸),流速为1.0ml/min,柱温为30℃。结果:芍药苷与迷迭香酸的质量浓度分别在4.20～42.00、4.10～41.00ng/μl范围内与各自峰面积积分值呈良好线性关系(r均为0.9999);精密度、重复性、稳定性试验的RSD均<2%;平均加样回收率分别为98.82%和97.80%,RSD分别为1.90%和1.23%(n均为9)。结论:本方法操作简便、结果可靠、重复性好,可用于安乳散结丸的质量控制。

OBJECTIVE： To establish a method for the simultaneous determination of paeoniflorin and rosmarinic acid in Anru sanjie pill. METHODS： The dual-wavelength HPLC was adopted. The analytical column was Agilent Eclipse pluse C18（250 min×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-0.1% phosphonic acid （20：80, V/V） at flow rate of 1.0 ml/min. The detection wavelength was set at 230 nm for paeoniflorin and 330 nm for rosmarinic acid. The column temperature was 30 ℃. RESULTS： The linear ranges of paeoniflorin and rosmarinic acid were 4.20-42.00 ng/μl and 4.10-41.00 ng/μl（r=0.999 9）. RSDs of precision test,reproducibility test and stability test were all lower than 2%. The average recoveries were 98.82% and 97.80%, and RSD were 1.90% and 1.23% （n=9）, respectively. CONCLUSION： The method is simple, reliable and has good repeatability. It can be used for quality control of the product.