黄连废弃物中生物碱含量的时空分布研究

Study on Spatial and Temporal Distribution of Alkaloids Contents in Coptis chinensis Wastes

目的:研究黄连废弃物(须根和茎叶)中小檗碱、黄连碱、巴马汀和表小檗碱等生物碱含量的时空分布情况。方法:先采用高效液相色谱(HPLC)法测定黄连废弃物中小檗碱在不同采收时间和不同部位中的质量分数,再以相对保留时间和峰面积比值计算样品中黄连碱、巴马汀和表小檗碱的质量分数。结果:黄连须根(全须)中生物碱质量分数变化不大,总体维持在2.7%左右。黄连茎叶中生物碱质量分数变化较大,其中黄连茎中生物碱质量分数冬季(0.9%)低于夏季(1.9%),但也分别高于黄连叶中冬季(0.7%)和夏季(1.0%)的质量分数。黄连近须和近茎(离黄连根≤4cm)中生物碱质量分数分别是远端的2倍以上。结论:黄连废弃物具有深入开发应用的价值。

OBJECTIVE： To study spatial and temporal distribution of alkaloids （berberine, coptisine, palmatine and epiberberine） contents in Coptis chinensis wastes （fibril, stem and leaf）. METHODS： The content of berberine in C. chinensis wastes with different harvesting time and different parts were tested by HPLC. The contents of coptisine, palmatine and epiberberine were calculated with relative retention time and peak area ratio. RESULTS ： There were no significant changes of alkaloids contents in C. chi- nensis fibrils without in stems and leaves （around 2.7% ）. Alkaloids content of C. chinensis stems and leaves showed great changes, and that of C. chinensis stems in winter （0.9%） was lower than in summer （1.9%） but higher than C. chinensis leaves in winter （0.7%） and in summer （1.0%）, respectively. Alkaloids contents in short-distance parts of C. chinensis fibrils and stems （less than 4 centimeters away from root） were two times more than in long-distance parts. CONCLUSIONS： There is a value for the deep development of C. chinensis wastes.