板蓝根药材中表告依春与fructopyrano-(1→4)-glucopyranose双糖体外抗病毒作用及量效关系研究

The in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-(1→4)-glucopyranose based on "deletion/increment" strategy

目的:研究板蓝根药材中表告依春、Fructopyrano-(1→4)-glucopyranose双糖(FG)体外抗流感病毒作用及量效关系。方法:荧光酶标法检测其对流感病毒神经氨酸酶(NA)的抑制作用;以半数抑制浓度(IC50)为指标,通过等效曲线分析表告依春和FG体外抗病毒的作用关系;分别向表告依春与FG的阴性样品中逐渐"增量",探讨表告依春和FG体外抗病毒作用的含量限量范围。结果:表告依春与FG样品合用的等效点在等效线下方,IC50,mix值小于IC50,add值;板蓝根中表告依春抗甲型流感病毒作用的最低、最优和最高含量点分别为82.5,225.0,289.7μg.mL-1,FG分别为138.2,450.0,618.9μg.mL-1。结论:板蓝根中表告依春与FG体外抗病毒作用具有协同性。本研究制定了板蓝根中抗病毒关键药效物质(表告依春、FG)的含量限度范围。

Objective ： To investigate the in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-（1→4）-glucopyranose （FG） from the traditional Chinese medicine Isatidis radix. Methods： Inhibitory effect of the target components on influenza virus neuraminidase （NA） was determined by fluorescent enzyme immunoassay. The 50% inhibitory concentration （IC50） was used as an indicator to research the relationship between epigoitrin and FG by equivalent curve analysis. The effective concentrations of epigoitrin and FG for in vitro anti-virus activity were determined. In the negative samples, the concentration range of target composition was gradually increased to obtain the key efficacy （ ＂incremental＂ ）. Results ： The equivalent point of epigoitrin and FG samples was located below the equivalent line, and the IC50,mix value was less than IC50,add. For anti-influenza A virus activity of the incremental samples of epigoitrin and FG, the effective concentrations were as follows： the lowest, optimum, and highest concentrations of epigoitrin were 82.5, 225.0 and 289.7 μg· mL^-1 ; those of FG were 138.2, 450.0 and 618.9 μg·mL^-1. Conclusion： The equivalent curve analysis shows that epigoitrin and FG exhibit cooperation. We have, for the first time, revealed the effective concentration range for in vitro anti-virus activity of epigoitrin and FG from Isatidis radix.