TcLr45凝集素类受体蛋白激酶基因克隆与序列分析

Cloning and Sequence Analysis of Lectin-like Receptor Kinases Gene in TcLr45

在小麦抗叶锈近等基因系TcLr45中,扩增出一条凝集素类受体蛋白激酶基因片段。以该序列为靶序列,进行3'和5'RCAE-PCR,获得该基因cDNA全长片段为2347 bp,该序列具有完整的开放阅读框,共编码730个氨基酸,命名为LecRK-LR45。经软件分析,LecRK-LR45具有典型的凝集素类受体蛋白激酶结构,属于L类型。聚类分析结果表明,LecRK-LR45与山羊草和小麦抗叶锈近等基因系TcLr34中的凝集素类受体蛋白激酶基因具有较近的亲缘关系,与玉米、拟南芥的亲缘关系较远。该基因的克隆为研究小麦中凝集素类受体蛋白激酶的功能奠定了基础。

A fragment of lectin-like receptor kinases gene was amplified in wheat leaf rust near-isogenic line TcLr45. The full length cDNA was 2347bp, which obtained using 3＇and 5＇RACE-PCR, baseing on the target sequence. This sequence had a full open reading frame （ORF） , encoding 730 amino acids, named as LeeRK-LR45. The LecRK-LR45 had a typical structure of lectin-like receptor kinases and belonged to L type. Cluster analysis result indicated that LecRK-LR45 had a closer genetic relationship with lectin-like receptor kinases gene of Aegilops tausehii and wheat（TcLr34） ,and farther with maize and arabidopsis. The cloning of this gene lied a foundation for researching the function of leetin-like receptor kinases in wheat.