摘要
南方锈病是我国玉米产区的主要病害,玉米抗病品种的利用是控制其为害的一条最为安全和经济的途径。但是,在我国当前的玉米育种中,所利用的玉米南方锈病基因多来自美国杂交种78599等。为寻找新的南方锈病抗病基因,本研究对CIMMYT自交系CML470的抗性进行了遗传分析。结果发现CML470的抗性由一个显性抗病基因(定名为RppC)控制,该抗病基因被定位于10号染色体短臂端部,位于SSR标记umc1380和umc1291之间,分别与两标记相距3.5 cM和8.8 cM。通过回交,并利用分子标记辅助选择,RppC被转移到了优良自交系昌7-2中。
Southern rust is one of the major diseases for maize in China. Deployment of resistant varieties is one of the most environment-friendliy and cost-saving methods. However,resistance genes used in current breeding programs mainly originated from the hybrid 78599. CML470 was a inbred line developed in CIMMYT and its inheritance of resistance to southern rust was studied to identify novel resistance genes. Genetic analysis showed that the resistance of CMIA70 was controlled by a dominant gene （ named as RppC） ,which was mapped to an interval on the terminal of chromosome 10 short arm flanked by closest SSR markers umc1380 and umc1291 with a distance of 3.5 cM and 8.8 cM,respectively. The resistance gene RppC was transferred into the elite line Chang 7-2 by backcross with the aid of molecular marker-assisted selection.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2013年第3期518-522,共5页
Journal of Plant Genetic Resources
基金
国家转基因生物新品种培育重大专项(2011ZX08003)
山东省自然基金项目(ZR2009DM007)
山东省良种工程"玉米抗病
专用种质创新利用研究"项目
山东省农业科学院博士基金项目(2007YBS006)
关键词
玉米
南方锈病
抗病基因
基因定位
标记辅助选择
Maize
southern rust
resistance gene
molecular mapping
marker assisted selection
