华南杂交稻亲本间产量配合力评价与分子标记鉴定

Evaluation and Molecular Marker Screening of Combining Ability for Yield among Major Parental Materials of Hybrid Rice in South China

选用华南地区广泛应用的8个杂交稻不育系与10个恢复系,按NCII设计配制2套不完全双列杂交组合61个,通过在湛江的2年种植试验,结合62对与产量性状QTL连锁SSR标记,对18个亲本的产量配合力进行综合评价和标记位点鉴定。结果表明:供试亲本间一般配合力存在较大差异,其中不育系珍汕97A、万金A和恢复系直龙、广恢3550的一般配合力相对较高;2套组合共检测出36个与亲本产量配合力显著相关的标记位点,其中RM1、RM216、RM231和RM542为2套材料所共有;检测出的杂合标记位点中,23个为增效标记位点,13个为减效标记位点,其中杂合标记位点RM231、RM209可使F1的单株粒重增加13.50%和16.43%。该结果可为华南地区杂交稻育种的亲本改良及选配提供理论指导。

Eight male sterile lines and ten restorer lines of hybrid rice in South China were crossed to develop two half-diallel mating sets designed according to NCH and sixty-one F1 were obtained. These F1 were planted in 2005 and 2006 respectively, and the combining ability for yield traits in its eighteen parents was evaluated and marker loci were identified by using sixty-two SSR markers related to functional QTLs. The results showed that there were obvious differences in general combining ability among these parent lines, and four parent lines including Zhenshan97A,WanjinA,Zhilong and Guanghui3550 had elite general combining ability. There were thirty-six SSR molecular loci associated with combining ability of yield traits, and marker loci RM1, RM216, RM231 and RM542 were detected in two sets of materials. Among them, twenty-three were effect-increasing locus while others were effect-decreasing locus. Heterozygous locus Of RM231 and RM209 were two marker loci of elite combining ability for yield with the maximum increasing effects, which increased 13.50% and 16.43 % of yield in F1, respectively. The results could be directly used for yield improvement and choice of parents for developing hybrid rice in South China.