11份割手密遗传多样性的SSR分析

Genetic Diversity Analysis for 11 Clones of Saccharum spontaneum L.

利用10对多态性丰富的SSR引物,以国家甘蔗种质资源圃中保存的14份具有代表性的割手密为对照,对新收集的云南省11份割手密(Saccharum spontaneum L.)资源进行多样性分析。结果共扩增出233条DNA谱带,与对照相比,新采集材料的多态性条带为207条,其中14条为特有条带,多态性条带比率为0.89。遗传相似性系数和UPGMA聚类分析表明,新采集的材料并没有单独聚为一类,而是比较分散,在相似性系数为0.64处作切割线,参试材料可分为3个类群:第Ⅰ类群主要由龙门割手密、河边村割手密和福建仙游1号组成;第Ⅱ类群中包含19份材料,其中新采集的样品有上岗割手密、他拉割手密、安乐割手密、勐根割手密、芒美割手密、贺海割手密、回落割手密、里拉割手密和曼亨割手密,对照材料主要包含了云南、四川、越南、老挝、泰国地区的割手密,其共同特点是均匀分布在内陆地区;第Ⅲ类群包括3个材料,分别是海南1号、海南92-2和广东化州割手密,其中不包含新采集的材料。而在相似性系数为0.654处作切割线又能将上述第Ⅱ类群分为较细的3个亚群。由此可见,新采集的11份割手密资源具有丰富的遗传多样性,与已收集的资源相比,具有一定的差异性。说明依靠云南高山峡谷等立体气候特点,分布着遗传差异显著的割手密无性系。

In this study, 11 original clones which had never been collected from Yunnan province as well as 14 control clones which had a better polymorphism from national nursery of sugarcane germplam were amplified with 10 SSR primers. As a result,a total of 233 bands were acquired. Out of these bands,207 were polymorphic bands and 14 were specific bands for the new collected clones compared to the control materials. The new collected materials could not be separated from the control by the genetic similarity coefficients and UPGMA cluster analysis. The 25 clones were divided into three groups at 0.64 of genetic similarity coefficient. The first group consisted of Longmen, River Village Saccharum spontaneum L. , and Fujian Xianyou No. 1. The second group consisted of 19 materials from inland areas,including 9 new gathering resources from Shanggang, Tala, Anle, Menggen, Mangmei, Hehai, Huiluo, Lila,and Manheng Saccharum spontaneum L. , and 10 control from Yunnan, Sichuan, Vietnam, Laos, and Thailand area. The third group included three control materials of Hainan No. 1, Hainan 92 - 2 and Huazhou of Guangdong S. spontaneum L.. The second group was divided into 3 sub-groups at 0. 654 of genetic similarity coefficient. These results indicated that the 11 new collections of S. spontaneum L. showed abundant genetic diversity in comparison with the control. Therefore, it supposed that there were striking genetic differences among these materials derived from the distinctive geographical distribution and climatic circumstances of Yunnan province.