栽培沉香遗传多样性的ISSR和AFLP分析比较

Comparative Analysis of Cultivated Aquilaria Lam.Using ISSR and AFLP

探讨2种分子标记技术在沉香属药用植物遗传多样性研究中的应用。用ISSR和AFLP分子标记分析了海南、云南、广东、广西等地17份沉香属植物的遗传多样性。14个ISSR引物、8对AFLP引物分别检测到119、919个位点,多态性位点百分率分别为73.95%、86.94%。由于AFLP标记具有较高的多态性位点检测效率,AFLP标记分析的遗传多样性参数高于ISSR。虽然基于Nei's遗传距离的聚类分析结果存在着一定的差异,但用Mantel检测对2种方法检测的遗传一致度进行相关性分析表明,二者之间存在着明显的相关性(r=0.7705,P=0.0003)。ISSR标记与AFLP标记均能应用于沉香属植物的遗传多样性研究。2种标记的研究结果均揭示出沉香属植物具有较高的遗传多样水平。

To discuss the application of 2 kinds of molecular markers in genetic diversity of cuhivated Aquilaria Lain.. The genetic diversity of 17 populations of Aquilaria Lam. from Hainan, Yunnan, Guangdong, Guangxi et al was analyzed using ISSR and AFLP. 14 ISSR primers and 8 AFLP primer combinations detected 119 and 919 loci of which the number of polymorphic loci was 88 （73.95%） and 799 （86.94%） respectively. The results of ISSR and AFLP revealed Aquilaria Lam. possessed higher genetic diversity. Because of different mechanisms of ISSR and AFLP, some differences existed in the results obtained. Genetic diversity parameters based on AFLP were higher than those based on ISSR, which was probably resulted from the higher detection efficiency of AFLP. Although some differences existed between the UPGMA dendrograms of ISSR and AFLP based on the Nei＇s genetic distances, Mantel test revealed obvious correlation between genetic identity matrix between ISSR and AFLP （ r = 0. 7705, P = 0. 0003 ）. Both methods could be used to analyzed the genetic diversity of Aquilaria Lam.