夏堇悬浮细胞培养与植株再生研究

Study on Cell Suspension Culture and Plant Regeneration of Torenia fournieri L.

以夏堇品种"小丑"的组培苗幼嫩叶片为试材,进行愈伤组织诱导、细胞悬浮培养及植株再生诱导的研究。结果表明:MS+1.0mg/L 6-BA+0.1mg/L 2,4-D的培养基能够使叶片诱导为生长迅速、质地疏松的愈伤组织。愈伤组织最适宜的悬浮培养条件为:MS+1.0mg/L6-BA+0.1mg/L 2,4-D液体培养基,20g/L的起始接种量以及100r/min的震荡培养。将继代3～4次的细胞悬浮颗粒转到1/2MS+1.0mg/L 6-BA+0.1mg/L NAA的固体再生培养基上培养,3周后可以诱导分化出芽,进而获得完整植株。悬浮培养有利于夏堇愈伤组织的再生,悬浮培养对提高以夏堇作为模式植物进行的相关研究具有重要的应用价值。

.Taking young leaves of Torenia fournieri ‘Clown＇ series as material, the callus induction, suspension culture and plant regeneration were studied. The results showed that MS medium with supplement of 1.0 mg/L 6-benzylamino purine （6-BA） and 0. 1 mg/L 2,4-dichlorophenoxyacetie acid （2,4-D） efficiently induced rapid-growing and loose callus. The obtained callus was taken as suspension culture material The most suitable conditions for the establishment of cell suspension culture were liquid MS medium with 1.0 mg/L 6-BA and 0. 1 mg/L 2,4-D,initial inoculum of 20 g/L,shaking speed of 100 r/rain. The suspended particles which were subcultured 3-4 times then cultured on half strength minerals of solid MS medium with 1.0 mg/L 6-BA and 0. 1 mg/L 1-naphthylacetie acid （NAA） for inducing regeneration. The buds came out in 3 weeks and later complete plants would be obtained. The suspension culture system had important application value in improving study which taking Torenia fournieri L. as a model plant.