苦荞发芽过程中不同部位的黄酮合成动态研究

Kinetic studies on flavonoids accumulation in different parts of tartary buckwheat during germination process

以苦荞种子为原料,研究其在发芽过程中胚根、胚轴和子叶中总黄酮以及芦丁、槲皮素的合成动态。结果表明:苦荞发芽过程中,其胚根、子叶中总黄酮含量在3～9d范围内增加幅度较大,增加至第9d时达到最大值,然后处于相对稳定水平;胚轴中的总黄酮含量逐渐降低。芦丁含量变化趋势和总黄酮含量变化趋势基本一致。胚根、子叶中的槲皮素含量在3～9d范围内逐渐降低,第9d后处于相对稳定水平;胚轴中的槲皮素含量逐渐升高。在同一发芽时间,总黄酮含量子叶中最高,其次是胚轴,胚根中含量最低。

Kinetic studies of rutin,quercetin,and total folavonoids accumulation in radicle,hypocotyl and cotyledon of tartary buckwheat during germination process were investigated. The result showed that total flavonoids content increased steadily during the first seven days. The highest flavonoids content was obtained on the 9th day,and maintained a stable level in the subsequent days. During the culture period,the fiavonoids content of hypocotyl decreased gradually. For the rutin accumulation,it was in accordance with the kinetics of the total folavonoids. The quercetin content in radicle and cotyledon of tartary buckwheat reduced gradually during the germination process. However,the quercetin accumulation increased steadily. Moreover,the total flavonoid content of cotyledon was higher than that of hypocotyl,and the flavonoid content of hypocotyl was higher than that of radicle at the same culture period.