摘要
目的:研究人参叶中总皂苷及人参皂苷Rg1、Re的含量测定方法。方法:采用紫外分光光度法,以人参皂苷Re为对照品,香草醛-冰醋酸-高氯酸为显色剂,测定人参叶总皂苷的含量;采用HPLC法测定人参皂苷Rg1、Re的含量。结果:人参叶中总皂苷、人参皂苷Rg1、人参皂苷Re分别在25.2～151.2μg、0.510～5.10μg、1.020～10.20μg范围内线性关系良好,平均回收率分别为99.53%(RSD=2.00%,n=6),99.02%(RSD=2.07%,n=6)和100.16%(RSD=2.67%,n=6)。结论:该方法重复性好,结果准确、可靠,为人参叶的质量控制提供了一定的参考。
Objective： To study the determination of the total ginsenosides and ginsenoside Rgl, Re in ginseng leaves. Methods： UV VIS spectrophotometry was used to determine the total ginsenosides, HPLC was used to determin ginsenoside Rgl and Re. Results ： The calibration curves were separately linear of the total ginsenosides and ginsenoside Rgl ,Re between 25.2%151.2%g,0. 510 5.10 9g, 1.020 % 10.20 9g. The average recoveries of the total ginsenosides and ginsenoside Rgl, Re were 99.53 % （RSD= 2.00 %, n=6）,99.02%（RSD=2.07%,n=6）and 100.16%（RSD=2.67%,n=6）, respectively. Conclusion：The method is accurate and reproducible. It is helpful to control the quality of ginseng leaves.
出处
《亚太传统医药》
2013年第5期49-51,共3页
Asia-Pacific Traditional Medicine
