常压下强电场辐射对国稻6号DNA甲基化的影响

Effect on DNA Methylation of Guodao 6 by Strong Electric Field Radiation at Atmospheric Pressure

目的:研究常压下强电场辐射对国稻6号DNA甲基化的影响。方法:常压下在相同时间下,以不同强度(和在相同强度下,以不同时间)辐射国稻6号种子,应用甲基化敏感扩增多态性(methylation sensitive amplification polymorphism,MSAP)技术,研究在不同辐射条件下,水稻基因组DNA甲基化的水平及差异。结果:所有强电场辐射的试验组甲基化水平都比未辐射的对照组有所降低,并与辐射的时间和强度有关。细胞DNA全甲基化、半甲基化扩增位点占总扩增位点的比例为:12.89%～13.62%,3.36%～4.63%,随着辐射强度和时间的增加有所降低。结论:说明经强电场辐射的国稻6号水稻基因组DNA甲基化较亲本发生了明显的变异,强电场能引起表观遗传变异,为研究其规律奠定了基础,也为探讨水稻甲基化对水稻生长调控的分子机理提供了帮助。

Objective： The strong electric field radiation under atmospheric pressure to the rice 6 the influence of DNA methyla- tion. Methods： Under atmospheric pr.essure at the same time, based on the different strength （and in the same strength, in different time） radiation Guodao 6 seed, the application ofDNA methylation sensitive amplification polymorphism （methylation sensitive amplification polymorphism, MSAP） technology, the research in different radiation condition, the rice genome DNA methylation level and difference. Results： All the strong electric field radiation treatment group methylation level than the control group did not radiation reduced, and ra-diation time and strength about. All cell DNA methylation, half methylation amplification sites accounted for the proportion of amplifica- tion sites for： 12.89% - 13.62%, 3.36% - 4.63%, with the increase of radiation intensity and time reduced. Conclusions： The strong elec- tric field radiation kingdom rice 6 rice genome DNA methylation is parent changed obviously variation that strong electric field can cause apparent genetic variation, and the rules for the research laid the foundation for discussion, and rice methylation on rice growth control molecular mechanism provides the help.