FEZ1和p16在宫颈上皮内瘤变中的表达及临床意义

Expression and clinical significance of FEZ1 and p16 in cervical intraepithelial neoplasia

目的 探讨FEZ1和p16在宫颈上皮内瘤变（CIN）中的表达及其相关性.方法 应用免疫组织化学方法检测93例CIN组织及10例正常宫颈组织中FEZ1、p16的表达情况.结果 在正常宫颈组织、CIN Ⅰ、CINⅡ、CINⅢ中,FEZ1的阳性表达率分别为90.0％（9/10）、67.9％（19/28）、36.0％ （9/25）、22.5％（9/40）,p16的阳性表达率分别为10.0％ （1/10）、32.1％（9/28）、76.0％（19/25）、92.5％（37/40）.FEZ1在CIN中的阳性表达率明显低于正常宫颈组织,p16则高于正常宫颈组织,差异均有统计学意义（P＜0.05）.二者在CIN Ⅰ与CINⅢ间阳性表达率比较差异均有统计学意义（P＜0.01）,在CINⅡ与CINⅢ间阳性表达率比较差异无统计学意义（P＞0.05）.FEZ1与p16在CIN中的表达呈负相关（r=-0.712,P＜0.01）.结论 FEZ1的异常低表达与p16的异常高表达参与了CIN的发生、发展过程,二者的联合检测可为CIN的诊断提供新的依据.

Objective To investigate the expressions and the relationship of FEZ1 and p16 in cervical intraepithelial neoplasia （CIN）.Methods The expressions of FEZ1 and p16 in 93 cases of CIN and 10 cases of normal cervical specimens were detected by immunohistochemistry.Results The positive expression rates of FEZ 1 were 90.0% （9/10） of normal cervical specimens,67.9% （19/28） of CIN I,36.0% （9/25） of CIN Ⅱ,22.5%（9/40） of CIN Ⅲ.The positive expression rates of p16 were 10.0%（1/10） of normal cervical specimens,32.1%（9/28） of CIN I,76.0%（19/25） of CIN Ⅱ,92.5%（37/40） of CIN Ⅲ.There were significant differences in the positive expression rates of FEZ1 and p16 between CIN Ⅰ and CIN Ⅲ （P 〈 0.01）,there was no significant difference in the positive expression rates of FEZ1 and p16 between CIN Ⅱ and CIN Ⅲ.The expression of FEZ1 and p16 was negatively correlation in CIN （r =-0.712,P〈 0.01）.Conclusion The abnormal high expression of p16 and abnormal low expression of FEZ1 in CIN may be involved in the occurrence and development of CIN,detecting the expressions of the two indexes may be helpful for clinical diagnosis.