卵巢癌组织中凋亡相关蛋白8的检测及其临床意义

The expression of thanatos-associated protein-8 in ovarian cancer tissue and its clinical significance

目的 检测卵巢癌组织中凋亡相关蛋白8（THAP-8）的表达,探讨其相关的临床意义.方法 选择卵巢癌患者86例,分别取卵巢癌组织和癌旁组织,采用荧光定量聚合酶链反应法检测THAP-8基因的表达量,免疫组织化学法检测THAP-8蛋白的表达量.结果 在卵巢癌组织和癌旁组织中THAP-8基因表达量分别为0.304±0.104和0.615±0.152,THAP-8蛋白表达量分别为30.16±12.34和67.34±22.37,卵巢癌组织均显著低于癌旁组织,差异有统计学意义（P＜0.01）.中、低分化卵巢癌组织中THAP-8基因和蛋白表达量显著低于高分化卵巢癌组织（0.216±0.085比0.457±0.124、24.22±9.21比46.24±17.52）,有淋巴结转移卵巢癌组织中显著低于无淋巴结转移卵巢癌组织（0.247±0.087比0.476±0.133、23.44±8.57比49.60±18.35）,临床分期Ⅲ、Ⅳ期卵巢癌组织中显著低于临床分期Ⅰ、Ⅱ期卵巢癌组织（0.232±0.114比0.424±0.125、25.64±8.12比44.78±16.84）,差异均有统计学意义（P＜0.01或＜0.05）.结论 THAP-8的失活参与了卵巢癌的发生、发展,可以作为卵巢癌患者肿瘤分级和预后评估的一个指标.

Objective To investigate the expression of thanatos-associated protein-8 （THAP-8） in ovarian cancer tissue,and explore its clinical significance.Methods A total of 86 patients with ovarian cancer were enrolled.The gene and protein expression of THAP-8 of the ovarian cancer tissue and adjacent tissue were detected by fluorescent quantitation polymerase chain reaction and immunohistochemistry.Results The gene expressions of THAP-8 of ovarian cancer tissue and adjacent tissue were 0.304 ± 0.104 and 0.615 ± 0.152.The protein expressions of THAP-8 in ovarian cancer tissue and adjacent tissue were 30.16 ± 12.34 and 67.34 ± 22.37.The gene and protein expressions of THAP-8 in ovarian cancer tissue were significantly lower than those in adjacent tissue （P〈 0.01）.The gene and protein expressions of THAP-8 in the low and middle degree of tumor differentiation of ovarian cancer tissue were significantly lower than those of the high degree of tumor differentiation of ovarian cancer tissue （0.216 ±0.085 vs.0.457 ±0.124 and 24.22 ± 9.21 vs.46.24 ± 17.52,P 〈 0.01 or 〈 0.05）.The gene and protein expressions of THAP-8 in lymph node metastasis of ovarian cancer tissue were significantly lower than those of no lymph node metastasis of ovarian cancer tissue （0.247 ± 0.087 vs.0.476 ± 0.133 and 23.44 ± 8.57 vs.49.60 ± 18.35,P 〈 0.01 or 〈0.05）.The gene and protein expressions of THAP-8 in clinical TNM pathologic Ⅲ,Ⅳ stage of ovarian cancer tissue were significantly lower than those of Ⅰ,Ⅱ stage of ovarian cancer tissue （0.232 ± 0.114 vs.0.424 ±0.125 and 25.64 ±8.12 vs.44.78 ± 16.84,P 〈0.05）.Conclusions The downregulation-expression of THAP-8 has a closed relationship with the development of ovarian cancer.The THAP-8 might be used as a potential marker to assess the degree of rumor grade and prognosis in ovarian cancer.