肉桂提取物醇质体透皮吸收研究

Percutaneous absorption of Cinnamon Cortex extract ethosomes

目的研究肉桂提取物醇质体经皮给药系统中提高药物渗透性的方法。方法以水蒸汽蒸馏法提取肉桂油,注入法制备不同浓度醇质体,以SD雄性大鼠皮肤作为媒介,Franz单室扩散池为体外模型,HPLC测定透过皮肤的肉桂酸含量。结果相比于20%肉桂醇质体,30%、40%和50%肉桂醇质体分别增渗1.31、1.69和2.06倍。氮酮、丙二醇、氮酮+丙二醇3组促渗剂相比于30%醇质体,分别促渗2.31、1.10、3.40倍。醇质体凝胶渗透速率大于30%醇质体和凝胶。结论提高醇浓度,加促渗剂,制备亲水凝胶的药剂学手段,能有效促进肉桂提取物的经皮渗透。

Objective To investigate the methods of enhancing the permeability in Cinnamon Cortex extract transdermal drug delivery system with ethosomes as a vector. Methods Cassia oil was extracted by steam distillation and injected into ethosomes with different concentration. The amount of cinnamic acid passing through the skin of SD male rats released from ethosomes was determined by HPLC under the model of Franz diffusion cells in vitro. Results Compared to CC, ethosome with 20% ethanol, the permeabilities of CC ethosomes with 30%, 40%, and 50% ethanol were increased by 1.31-, 1.69-, and 2.06-fold, respectively. Compared to CC ethosome with 30% ethanol, the permeabilities were increased by 2.31-, l. 10-, and 3.40-fold, with the penetration enhancer of Azone alone, propylene glycol alone or both, respectively. The permeability of CC ethosome gel was higher than that of CC ethosome and cassia oil gels. Conclusion The tmnsdermal permeability of CC extract is enhanced by rising the concentration of ethanol, adding penetration enhancer, and preparing hydrogel.