4-羟基环孢菌素A衍生物[γHyMeLeu4]CyA生产菌的接合转移

Conjugation of [γHyMeLeu^4] CyA-Producing Strain

将染色体整合型糖多孢红霉菌表达载体pZMW-tsr转入大肠杆菌ET12567(pUZ8002);以其作为供体,采用接合转移的方法将硫链丝菌素抗性基因tsr转入4-羟基环孢菌素A衍生物[γHyMeLeu4]CyA生产菌野野村菌CYA4OH。PCR结果表明,tsr基因已经整合到CYA4OH基因组上。研究表明pZMW载体能够在稀有放线菌野野村菌中有效表达外源基因。

The recombinant vector pZMW-tsr, which could be integrated into S. erythraea chromosome,was transformed to Escherichia coli ET12567 （pUZ8002）. Gene tsr was transformed to the [γHyMeLeu4]CyA-pro- ducing strain Nonomuraea sp. CYA4OH by conjugation method using Escherichia coli ET12567 （pUZS002, pZMW-tsr） as a conjugal donor. PCR Identification showed that gene tsr was integrated into the chromosome of CYA4OH. It was confirmed that pZMW could express exogenous genes effectively in rare actinomycete Nono- rnuraea sp..