p53蛋白在食管癌前疾患及食管癌组织中的表达

Expressions of p53 Protein in Different Severe Esophageal Precancerious Lesions and Carcinoma

目的 观察食管癌高发区人群各种食管癌前疾患及食管癌组织中p5 3蛋白的表达并探讨其可能的机制。方法 应用免疫组化方法检测 2 18例食管不同阶段病变 (基底细胞增生、不典型增生、食管癌 )中p5 3的表达。结果 p5 3阳性表达检出率 ,在基底细胞增生为 41 84% ,不典型增生为 73 85 % ,食管癌为 75 %。基底细胞增生病例中有 86 44%为“ +”阳性 ,不典型增生病例“ +”阳性占5 8 33 % ,“ + +”以上阳性占 41 6 7% ,食管癌病例中p5 3表达水平均在“ + +”以上 ,且 5 5 5 6 %的病例为“ + + + +”阳性强表达。结论 在食管癌前病变期特别是良性增生阶段就存在p5 3蛋白的表达 ,这可能是由于损伤诱导的细胞内野生型p5 3蛋白表达 ,不可将p5 3蛋白的阳性表达均视为恶性转化的必然趋势。

Objective To observe the expressions of p53 protein in different esophageal precancerious lesions and carcinoma in symptom free subjects who were from an area with high esophageal cancer incidence and to study possible mechanism.Methods The expressions of p53 protein in 218 cases of different esophageal lesions(basal cell hyperplasia,dysplasia and carcinoma) were detected by immunohistochemistry.Results The positive rate of p53 immunostain were 41.84%,73.85% and 75%,respectively in basal cell hyperplasia,dysplasia and carcinoma.Of p53 immunostainpositive cases in basal cell hyperplasia,86.44% was “+” positive,in dysplasia “+” positive accounted for 58.33% and over “++”was 41 67%. No cases of “+” positive was found and “++++” intensity stain was 55.56% in carcinoma.Conclusions Expressions of p53 protein were detected in early stage in the development of human esophageal cancer,specially in benign hyperplasia phase.It is likely that cellular insults can induce wild type p53 expression in cells.It is arbitrary that p53 protein expression is necessarily indicative of malignance.