肉桂酸诱导人肺腺癌细胞恶性表型逆转和分化作用

Studies on Cinnamic Acid Inducing the Reversion of Malignant Phenotypes and Differentiation of Human Lung Adenocarcinoma Cells

探讨肉桂酸对人肺腺癌A5 49细胞诱导分化作用及可能机制。方法 应用 2mmol/L终浓度肉桂酸作用于人肺腺癌A5 49细胞 ,观察体外生长增殖情况、双层软琼脂集落形成率并用流式细胞仪测定细胞周期及p5 3蛋白表达。结果 细胞体外生长速度减慢 ;双层软琼脂集落形成减少 ;细胞DNA合成能力降低 ;细胞周期出现向G1/G0期移行的特征性动力学改变 ;p5 3蛋白表达减少。结论 肉桂酸通过激活人肺腺癌细胞分化相基因表达启动细胞分化并达到恶性逆转 ,是一种有应用潜力的诱导分化剂。

Objective To study the differentiation effect and possible mechanism of cinnamic acid (CINN) on human lung adenocarcinoma A549 cells.Methods A549 cells were treated by CINN (2 mmol/L),The cell growth rate was determined by cell counting,and its viability was determined by trypan blue exclusion assay.And the flow cytometry (FCM) was used to analyze the cell cycle and to detect p53 protein.All experiments were done in triplicate.Results After A549 cells were incubated with CINN,the cell proliferation was markedly inhibited;The cloning efficiency on soft agar of the CINN groups was less than that of the control group;CINN may inhibit the synthesis of DNS and mitosis of A549 cells,and make cell growing in the G1/G0 phase stasis;The CINN group had less mp53 protein than the control group and the difference between them was significant ( P <0 05).Conclusions CINN could significantly inhibit the proliferatioin of A549 cells,and has certain effect on the induction of differentiation which suggest that CINN may have potential use as a DA in lung adenocacinoma intervention.The decrease of mp53 protein after CINN treatment suggest that mp53 involves in the process of induction of differentiation on A549 by CINN.