前列腺癌细胞雄激素受体基因CAG重复序列改变的初步研究

Changes of CAG Repeat Length within the Androgen Receptor Gene in Prostate Cancer Cells

目的 了解前列腺癌细胞雄激素受体 (AR)基因 CAG重复序列有否改变。方法 显微镜下分别取 11例前列腺癌标本的肿瘤细胞及非肿瘤细胞 ,提取 DNA,经 PCR扩增后 ,用变性胶电泳测得肿瘤细胞和非肿瘤细胞 DNA含 AR基因 CAG重复序列片段的相对长度。结果 有 3例 (2 7.3% )前列腺癌细胞 AR基因 CAG重复序列较非肿瘤细胞短。其余 8例前列腺癌细胞 AR基因 CAG重复序列与非肿瘤细胞相同。结论 一些病例的前列腺癌细胞 AR基因 CAG重复序列可发生变化 ,其变化与前列腺癌的发生、发展和对抗内分泌治疗的关系尚待进一步的研究。

Objective\ To investigate the changes of CAG repeat length within the androgen receptor (AR) gene in prostate cancer cells Methods\ Tumor cells and non-tumor cells were collected respectively under microscope from the sections of 11 cases of prostate cancer Genomic DNA was amplified by PCR and the relative CAG repeat lengths within the AR gene in both tumor and non- tumor cells were detected by denaturing gel electrophoresis Results\ In 3 of the cases (27 3%),the CAG repeat length in tumor cells were shorter than that in non-tumor cells and in the other cases,tumor cells have the same CAG repeat length as non-tumor cells Conclusions\ CAG repeat length within the AR gene may change in some prostate cancer cells