黑曲霉ZJUQH产α-半乳糖苷酶的固体发酵培养基优化研究

Optimization by Response Surface Methodology of Solid-state Fermentation Medium for α-Galactosidase Production by Aspergillus niger ZJUQH

通过响应面方法优化固体发酵培养基,以提高黑曲霉ZJUQH产α-半乳糖苷酶的能力。在前期研究的基础上,通过部分因子试验对蛋白胨、Na2HPO4·12H2O、料液比和接种量进行系统考察,筛选出两个影响较显著的因素料液比和蛋白胨添加量,然后通过中心组合试验进一步优化,建立以α-半乳糖苷酶酶活力为响应值的二次回归方程模型,获得了最优的固体发酵培养基组成:蛋白胨0.6g、Na2HPO4·12H2O 0.08g、料液比1:2.44(m/V)接种量1.5mL/5g。在该优化的培养条件下,固态发酵6d产α-半乳糖苷酶的酶活达到(77.21±2.01)U/g,比优化前(蛋白胨0.4g、Na2HPO4·12H2O 0.08g、料液比1:3、接种量1.5mL/5g)培养得到的酶活力最高值(49.05±2.11)U/g提高57.41%。

In order to enhance the production of α-galactosidase by Aspergillus niger ZJUQH,response surface methodology was applied to optimize the solid culture medium.A fractional factorial design was used to investigate the main factors affecting α-galactosidase yield.The results showed that two factors played important roles in the production of α-galactosidase.Central composite design and response surface analysis were applied to establish a second-order regression equation model for the yield of α-galactosidase.At last,the optimal fementation condition were obtained by response surface analysis as follows： peptone 0.6 g,Na2HPO4.12H2O 0.08 g,ratio of solid to water 0.41 and inoculum quantity 1.5 mL/5g.Under the optimal conditions,the activity of α-galactosidase reached（77.21±2.01） U/g,which was increase by 57.41% compared with（49.05 ± 2.11） U/g before the oprimization,that is,peptone 0.4 g,Na2HPO4.12H2O 0.08 g,ratio of solid to water 0.33 and inoculation quantity 1.5 mL/5 g.