介绍一种测定食品中山梨酸、苯甲酸的新方法

New method for determination of sorbic acid and benzoic acid in food

目的:建立一种测定食品中防腐剂山梨酸、苯甲酸的新方法。方法:利用HP-INNWAX毛细管气相色谱法测定食品中山梨酸、苯甲酸。结果:在所给出的实验条件下,方法的回收率为99%;山梨酸、苯甲酸含量在150μg/ml时,RSD分别为5.8%和4.3%(n=6);在0μg/ml～200μg/ml范围内方法校准曲线相关系数r分别为0.9999和0.9993;方法的最低检测浓度分别为13 mg/kg,33 mg/kg。结论:本法准确、可靠、简便、快速、检出限低,适用各类食品中山梨酸、苯甲酸的同时分析测定,完全能够取代GB/T5009.29-2003的第一法。

Objective：To establish a new method for determination of food preservatives sorbic acid and benzoic acid in food.Methods： HP-INNWAX capillary gas chromatography was developed for the determination of sorbic acid and benzoic acid in food.Results： Under the given experimental conditions,the recovery of the method was 99%;When the contents of sorbic acid and benzoic acid were at 150 μg/ml,RSD were 5.8% and 4.3% respectively（n=6）;In the range of 0 μg/ml^200 μg/ml,the calibration curve correlation coefficients of the method were 0.9999 and 0.9993;The minimum detectable concentrations were 13 mg/kg and 33 mg/kg for sorbic acid and benzoic acid respectively.Conclusion： This method is accurate,reliable,simple,fast with low detection limit,it is suitable simultaneous determination of sorbic acid and benzoic acid in all kinds of food,completely a replacement of the first method in GB/T5009.29-2003.