摘要
目的:比较三种不同配方的细胞生长液培养MDCK细胞效果。方法:使用不同配方的细胞生长液,在其他培养条件相同的情况下培养细胞并比较培养效果。结果:使用优化配方的细胞生长液培养MD-CK细胞,在细胞贴壁情况和细胞形态方面优于其它两种配方,有统计学意义。结论:优化后细胞生长液更适合本实验室流感病毒分离的实际工作需要。
Objective:To compare the culture effect of MDCK cells by cell culture medium with different formulas.Methods: Under the same culture condition,the MDCK cells were cultured in three different formulas of culture medium,then the results were compared.Results: The optimized culture medium has better performance in MDCK cells culturing than the other two formuals.Conclusion: An optimized culture medium for the cultivation of MDCK cells was proven to better meet the practical need in laboratory.
出处
《中国卫生检验杂志》
北大核心
2013年第4期860-861,共2页
Chinese Journal of Health Laboratory Technology
基金
黑龙江省卫生厅科研项目(2011-491)