支气管哮喘患者Th17及Tc17细胞的表达及意义

The prevalence of blood Th17 and Tc17 cells in patients with bronchial asthma

目的:探讨外周血Th17细胞、Tc17细胞在支气管哮喘患者中的表达及临床意义。方法:选取37例轻度和23例中重度急性支气管哮喘患者及26例哮喘缓解患者作为研究对象,采用流式细胞术检测Th17细胞及Tc17细胞百分率、Treg细胞的百分率,ELISA方法测定IL-17的表达。结果:轻度、中重度急性哮喘组外周血中Th17及Tc17细胞百分率,IL-17的含量均高于缓解组及正常对照组(P<0.01),中重度哮喘组高于轻度哮喘组(P<0.01)。Tc17细胞在治疗缓解组中表达高于正常对照组(P<0.01)。结论:Tc17细胞及Th17/Tc17联合检测可能成为哮喘发作期的检测指标,并有望为哮喘的治疗提供新的靶点。

Objective：To investigate the expression of Th17 and Tc17 cell in patients with bronchial asthma and its significance.Methods： Thirty-seven mild bronchial asthma patients,23 moderate bronchial asthma patients,26 asthma patients in alleviated period and 18 healthy donors were enrolled.Percentage of peripheral blood Th17 cells and Tc17 cells and Treg cells were determined by flow cytometry.IL-17 levels were measured by ELISA.Results： The percentage of Th 17,Tc17 and the level of IL-17 in patients with mild asthma and moderate-severe were higher than those in asthma patients in alleviated period and healthy donors（P0.01）.The percentage of Tc17 in patients with alleviated period asthma was higher than healthy donors（P0.01）.Conclusion： The combination of Tc17 cells and Th17/Tc17 could be used as detection index of bronchial asthma and expected to be provide new target point for asthma treatment.