婴幼儿配方奶粉中克雷伯菌的最低检出限与回收实验研究

Study on recovery and lowest detection limit of Klebsiella determination in infant formular milk powder

目的:对研制的改良麦康凯培养基(MACI)的回收率和克雷伯菌在该培养基上的最低检出限进行研究。方法:选择产酸克雷伯菌ATCC13182和肺炎克雷伯菌ATCC10031为试验菌株,以NA培养基为对照,采用涂布法测试MACI培养基的回收率;试验菌株稀释成不同浓度后分别加入到已知无肠杆科细菌的婴幼儿配方奶粉中,涂布法测试克雷伯菌在MACI培养基上的最低检出限。结果:两种菌株在MACI培养基上的回收率分别为89.90%和93.66%,加标实验菌株的最低检出限为40 cfu/ml。结论:MACI不仅可快速准确地分离克雷伯菌,而且试验菌株在此培养基上的回收率及最低检出限可以达到比较理想的结果。

Objective：To evaluate the recovery and the lowest detection limit of Klebsiella determination in infant formular milk powder by using domestic-developed MACI as medium.Methods： ATCC13182 and ATCC10031 were chosen as testing bacterial strains with NA as controls,recoveries were evaluated by coating them on MACI cultures and the lowest detection limits were obtained by determining ATCC13182 and ATCC10031 concentration in infant formular milk powder.Results： Recoveries of two bacterial strain determination method were 89.90% and 93.66% respectively and the lowest detection limit of method was 40 cfu/ml.Conclusion： Klebsiella can be separated easily and satisfactory recovery as well as low detection limit can be achieved by employing MACI as Klebsiella culture medium.