结核抗原快速检测在艾滋病合并结核病诊断中的应用

Application of tuberculosis antigen detection in the diagnosis of co-infection of AIDS and TB

目的:通过与传统的结核病实验室诊断方法(直接涂片抗酸染色、结核培养)的比较,评价结核抗原检测在结核病快速诊断中的应用价值。方法:对48例临床确诊的艾滋病合并结核病患者的痰标本进行抗酸染色检测和培养,外周血标本用于结核培养,并将分离的外周血血清用于检测结核抗原,对三种方法的结果进行分析比较。结果:艾滋病合并结核病患者的痰检组和血培养的阳性率分别是21.42%(9/42)、4.35%(2/46),特异性分别为100%和50.0%。结核抗原血清学检测组阳性率和特异性分别为31.11%(14/45)、100%。单个样本阳性率表明,检测血清中结核抗原方法的阳性率最高,阳性率最低的是血培养检测组。结论:血清抗原检测结核分枝杆菌是一种简单、快速、敏感性高、特异性强的结核分枝杆菌辅助诊断方法。

Objective：By comparing with traditional TB laboratory diagnostic methods（direct smear microscopy,culture of Mycobacterium tuberculosis）,to evaluate the application value of tuberculosis antigen detection in rapid diagnosis of tuberculosis.Methods： The sputum specimens from 48 AIDS patients infected with TB were detected by smear microscopy,the peripheral blood specimens were collected for tuberculosis culture,and the serum was isolated to detect the tuberculosis antibody.Then the results of the three methods were compared.Results： The positive rates of sputum smear and blood culture of AIDS patients infected with TB were 21.42%（9/42）,4.35%（2/46）,specificity were 100% and 50% respectively.The tuberculosis antigen serological testing positive rate and specificity were 31.11%（14/45）,100%.The positive rate of a single sample showed that the serum tuberculosis antigen method has highest positive rate,and blood culture testing group has the lowest positive rate.Conclusion： Serum antigen detection of Mycobacterium tuberculosis is a simple,rapid,high sensitive and specific method for auxiliary diagnosis of mycobacterium tuberculosis.