食管鳞癌P53蛋白和增殖细胞核抗原表达的临床价值

The Expression of P53 Protein and Proliferating Cell Nuclear Atigene in Squamous Cell Carcinoma of Esophagus

采用SP免疫组化方法 ,检测 137例食管鳞癌手术标本癌组织P5 3蛋白、增殖细胞核抗原(PCNA)和同例标本切缘食管粘膜上皮P5 3蛋白的表达 ,均采用石蜡切片。其中早期癌 6例、浸润癌131例。全组病例中癌组织P5 3蛋白表达的阳性率为 6 8.6 % ,过度表达为 36 .5 %。同一病人配对 131例标本切缘正常食管粘膜上皮P5 3蛋白表达的阳性率为 2 5 .2 % ,过度表达胃 6 .1%。 6例早期食管癌P5 3阳性表达率为 10 0 % ,过度表达率为 6 6 .7%。癌组织中PCNA阳性表达率 94.9% ,过度表达为73.7%。鳞癌P5 3蛋白过度表达与肿瘤分级有统计学显著性意义 ,P <0 .0 0 5。PCNA的过度表达与肿瘤分级没有统计学上意义 (P >0 .0 5 )。本研究提示 ,食管鳞癌P5 3蛋白的过度表达 ,不仅有助于食管早期癌的诊断 。

Expression of P53 protein and proliferating cell nuclear antigene(PCNA) in conseccutive 137 cases surgical operation samples of squamous cell carcinoma of esophagus and 131 cases normal esophageal epithelium was sudied with immunohistochemical SP method from June 1996 to December 1997,68.6% positive and 36.5% overexpression of P53 protein were shown in cancer tissue, and 25.2%, 6.1% respectively for normal esophageal epithelium. In 6 cases early esophagus carcinoma,100% positive and 84.6% overexpression of P53 protein were shown in cancer tissue. PCNA shown 94.6% positive and 73.7% overexpression. Marked statistic significantion was found among P53 protein expression and tumor classify grade(P< 0.05) , but PCNA No statistic significance (P>0.05). The study showed the overexpression of P53 protein. could help to diagnosis esophagus carcinoma and estimate its occurrence and prognosis.