基于双层纳米金修饰传感器的应用研究

Study of biosensor and application of double layer of gold nanoparticles modified glassy carbon electrode

制备了由聚番红花红和DNA固定的双层三维分布的纳米金修饰的玻碳电极,在此基础上研究了多巴胺(DA)在此修饰电极上的电化学行为,发现对DA的氧化,双层纳米金比单层的纳米金修饰的玻碳电极更能够起到明显的电催化作用。利用差分脉冲法(DPV)考察了DA测定的优化条件,并发现其浓度在1.0×10-8～1.0×10-6mol/L范围内与峰电流呈良好的线性关系。该电极用于实际样品的测定,结果满意。

The three-dimensional distributed gold nanaoparticles （GNPs）, which were anchored with poly（safranine T） and DNA, modified electrode was prepared. Based on the above, the electrochemical behavior of dopamine （DA） at this modified electrode was investigated. It was found that the double layer of GNPs modified electrode shows a better electrocatalytic activity for the oxidation of DA than that at single layer of GNPs. The optimum conditions of DA determination were studied by differential pulse vohammetry （DPV） and it was found that the linear relationship between peak current and concentration was in the linear range of 1.0×10^-8～1.0×10^-6mol/L. The modified electrode can be applied to the determination of DA in the practical injection samples with satisfactory results.