肝血管平滑肌脂肪瘤中肌样细胞的免疫组化观察

IMMUNOHISTOCHEMICAL STUDY OF MYOID CELL IN HEPATIC ANGIOMYOLIPOMA

目的 :探索肝血管平滑肌脂肪瘤 (AML)主要细胞成分的特异免疫标记 ,以提高诊断水平。方法 :对 1例肝AML的多块组织做连续切片 ,以标记上皮、非上皮、神经组织、单核巨噬细胞、黑色素等 1 5种抗体做免疫组化染色 ,并以HMB 45与α SMA和CD 68做双重标记染色。结果 :易致误诊的肌样细胞 (MC)呈HMB 45阳性反应 ,对α SMA呈阴性反应。结论 :AML中的肌样成分并非一般平滑肌细胞 ,这为确诊提供了依据。

Objective:To better define the morphologic characteristic of myoid cell(MC) with immunohistological staining,to improve the level of correct diagnosis of hepatic angiomyolipoma(AML).Methods:Fifteen kinds of monoclonal antibodies for epithelial,nonepithelial,neuronal tissues,mononuclear phagocytic cell and melanin were done.HMB 45 antibody was used with double staining for smooth muscle actin(α SMA) and CD 68 respectively.Results:MC which often lead to misdiagnosis showed a positive reaction for HMB 45 and negative for α SMA.Conclusion:The MC cells did not originally come from smooth musle cells.