CD_(15)、CD44v6和nm23H1的mRNA在鼻咽癌中表达的临床意义及相关性

Clinical significance and correlation of the expression of CD_(15) mRNA、CD44v6 mRNA and nm23H1 mRNA in nasopharygeal carcinoma

目的 探讨CD15mRNA及其蛋白、CD44v6和nm2 3H1的mRNA表达与鼻咽癌 (NPC)临床意义及其相关性。方法 应用免疫组化和原位杂交CSA方法 ,检测 6 5例NPC中CD15mRNA及其蛋白、CD44v6和nm2 3H 1的mRNA表达 ,结合临床病理指标及随访病例进行研究分析。结果 在NPC中CD15mRNA及其蛋白、CD44v6和nm 2 3H1的mR NA表达阳性率分别为 44.6 %、40 %、47.7%和 5 2 .3 %。CD15mRNA及其蛋白、CD44v6mRNA阳性表达和nm2 3H1mR NA阴性表达与NPC原发病灶分期和淋巴结转移均呈正相关 ,患者 5年内复发转移率高 (P <0 .0 1) ,5年存活率低 (P <0 .0 5 )。CD15mRNA与其蛋白表达和CD44v6mRNA表达呈正相关 ,与 2 3nmH1mRNA表达呈负相关。结论 (1)NPC中CD15mRNA表达与CD44v6和nm2 3H1的mRNA表达具有调节和负调节的协同作用。 (2 )CD15、CD44v6和nm 2 3H1的mRNA均可作为预测NPC转移、复发和评估预后的重要生物学指标。

Objective To invetigate the clinical significance and correlation of the expression of CD15 mRNA and the protein、CD44v6 mRNA、nm23H1 mRNA in nasopharygeal carcinoma(NPC).Methods CSA immunohistochemistry and in situ hybridization were used to detect the expression of CD15 mRNA and protein,CD44v6 mRNA、nm23H1 mRNA in 65 cases of nasopharygeal carcinoma and the study was comibined with analysis of clinical pathology index and follow up survey of NPC cases.Results In NPC tissues,the positive rate of CD15 mRNA and protein、CD44v6 mRNA、nm23H1 mRNA were 44.6%、40%、47.7%、52.3%,respectively.The positive expression of CD15 mRNA and protein、CD44v6 mRNA and the negative expression of nm12H1 mRNA were positively correlated with the staging of primary NPC and its lymph node metastasis,these patient had a high recurrence and metastatic rate (P<0.01) and a low survival rate(P<0.05) in five years.The expression of CD15 mRNA and protein was positively correlated with that of CD44v6 mRNA,and negatively with that of nm23H1 mRNA.Conclusions (1)The expression of CD15 mRNA has a synergistic action in positive and negative regulation with that of CD44v6 mRNA and nm23H1 mRNA in NPC.(2)These three kinds of mRNA are important pathological biology indexes to predict the metastasis and recurrence of NPC and evaluate its prognosis. [