异博定增加化疗中卵巢癌细胞凋亡的研究

Study on the synergetic effect of isoptin on induced cellular apoptosis of ovarian carcinoma by chemotherapy

目的 探讨异博定对促进化疗药物诱使卵巢癌细胞增加凋亡因子表达的作用。方法 从 48例首次手术及 17例复发卵巢癌患者腹水中提取富含癌细胞的悬液 ,应用三种荧光单克隆抗体P170、CD95及Apo2 .7标记 ,经流式细胞仪检测。结果 应用异博定化疗后癌细胞CD95及Apo2 .7表达含量均高于对照组 ;异博定对癌细胞P170表达含量无明显影响 ;复发组癌细胞P170表达含量显著高于初发手术组 ;化疗可使外周血淋巴细胞CD95及Apo2 .7表达含量增加。结论 本研究提示异博定具有加强化疗药物增加卵巢癌细胞凋亡的作用 ,特别是对耐药性患者具有重要的临床应用价值。

Objective To explore the role of calcium antagonist isoptin on the cellular Mdrl product(P170) and apoptosis of ovarian carcinoma in chemotherapy.Methods The ascites cancer cells(ACC)were collected in 48 primary and 17 recurred ovarian cancers,immunologically marked by three kinds of fluorescent monoantibodies P170,CD95 and Apo2.7 and then determined by flow cytometry.Results isoptin did not affect the P170 expression contents in ACC;The CD95 and Apo2.7 expression contents on ACC were statistically improved in isoptin group than in non isoptin one(P<0.05);The P170 contents were statistically improved in recurred group than that in primary one(P<0.05);The CD95 and Apo2.7 contents of peripheral lymphocyte were also improved in isoptin group.Conclusions The calcium antagonist isoptin obviously improves the apoptotic rate of ACC in ovarian carcinoma in chemotherapy and has important clinical significance. [