胃粘膜脱落细胞端粒酶活性研究

Detection of telomerase activity in exfoliated cells of the stomach

目的 探讨胃镜直视下刷取胃粘膜脱落细胞中端粒酶活性对胃癌的诊断价值。方法 采用TRAP PCR ELISA法对 5 6例胃组织和胃粘膜刷落细胞进行端粒酶活性的分析。结果 胃癌组端粒酶活性水平 (平均A值 0 .745 )明显高于胃炎组 (平均A值 0 .0 6 8)。端粒酶活性水平在胃刷落细胞中与组织的是相近的。端粒酶在胃癌组的阳性率为90 % ,明显高于胃刷落细胞涂片的阳性率 (6 6 .7% )、血CEA的阳性率 (5 6 .7% )。同时还测得 75 %的胃粘膜不典型增生者端粒酶活性水平也显著升高。结论 胃镜直视下刷取胃粘膜脱落细胞中端粒酶活性测定可作为诊断胃癌有效而敏感的方法。

Objective To investigated the diagnostic significance of telomerase activity in the exfoliated cells of stomach obtained from endoscopic brushing.Methods The technique of TRAP PCR ELLSA was employed to detect telomerase activity in 56 cases with gastric lesions.Results Telomerase activity of group of carcinoma of the stomach of (average A 0.745)was significantly higher than that of gastritis group (average A 0.068).Telomerase activity in exfoliated cells of the stomach was simillar to that in gastric tissue.Positive rate of telomerase activity detected in carcinoma of the stomach group(90%) was significantly higher than that of cytological examination(66.7%)、blood CEA(56.7%).75% of the cases with dysplasia were found to be telomarse activity positive.Conclusions Detection of telomerase activity in the exfoliated cells obtained from endoscopic brushing may be an effective and sensitive method in the diagnosis of carcinoma of the stomach. [