摘要
目的:改进《中国药典》2010年版附录芳香第一胺鉴别试验方法,避免假阳性结果。方法:在滴加碱性β-萘酚试液前加入脲溶液中和多余的亚硝酸,避免多余的亚硝酸与β-萘酚反应生成猩红色的1-亚硝基-2-萘酚而干扰鉴别试验现象的观察。结果:脲溶液可以有效中和反应过程中多余的亚硝酸,使反应后的溶液颜色不再干扰沉淀颜色的辨识。结论:本方法可以避免鉴别试验出现假阳性结果。
Objective: To improve the identification test of aromatic primaryamines in Appendix of Chinese Pharmacopoeia (2010 Edi- tion) and avoid false positive results. Method: Add urea solution to neutralize the excess nitrous acid before adds alkaline/3-naphthol test solution for the purpose of avoiding the interference since excess nitrous acid could react with/3-naphthol and generate 1-Nitreso-2- naphthol, which is scarlet in color. Result: The urea solution can neutralize the excess nitrous acid and the color of solution get from the reaction will no longer disturb the identification of precipitation. Conclusion: This method could avoid false positive results in the identification test of aromatic primary amines in Appendix of Chinese Pharmacopoeia (2010 Edition).
出处
《中国药品标准》
CAS
2013年第2期129-130,共2页
Drug Standards of China
关键词
芳香第一胺鉴别试验
假阳性
脲
identification of aromatic primary amines
false positive results
urea