心肌梗死患者血管紧张素转换酶基因型分析

Analysis of angiotensin converting enzyme gene polymorphism in myocardial infarction

目的 研究心肌梗死与血管紧张素转换酶 (ACE )基因多态性的关系。方法 应用PCR技术扩增病人外周血ACE基因第 16内含子目的片段 ,琼脂糖凝胶电泳确认等位基因 ,计算各组基因型频率。结果 病例组和对照组ACE基因多态性分布频率 ,DD型分别为 41 4%和 2 2 0 %、ID型分别为 31 0 %和 41 5 %、II型分别为 2 7 6 %和 36 6 % ,病例组DD型高于对照组 ,χ2 =4 2 1,P <0 0 5。

Objective: To research inte the relationship between deletion polymorphism in angiotensin converting enzyme (ACE) and myocardial infarction Methods: A 287 bpI/D polymorphism in intron 16 of the ACE gene was examined by polymerase chain reaction in a cross sectional study of 41 subjects and 58 patients with myocardial infarction. Results: The frequencies of DD genotype were 41 4% and 22 0%, ID genotype, 31 0% and 41 5%, II genotype, 27 6% and 36 6% in myocardial infaction and normal human respectively. The frequency of DD genotype in patients was higher than that in the control group, χ 2=4 21,P<0 05. There is no significant difference of DD genotype between male and female. Conclusion: It suggests that the DD genotype of ACE might be an important potent risk factor for myocardial infarction.