新型淀粉纳米囊人工红细胞有效性初步研究

Evaluation of Nanosized Cationic Amylose-encapsulated Bovine Hemoglobin

目的:采用季铵盐阳离子淀粉为囊材包裹牛血红蛋白,制备成纳米级的新型季铵盐阳离子淀粉人工红细胞(NanosizedCationic Amylose-encapsulated Hemoglobins,NCAHbs)并评价此新型人工红细胞的有效性。方法:应用大鼠重度失血性休克模型,将50只健康SD大鼠随机分成5组:休克对照组(Control组,n=10)、淀粉纳米囊人工红细胞组(NCAHbs组,n=10)、Ringer乳酸盐溶液组(Ringer's组,n=10)、羟乙基淀粉组(HES组,n=10)和全血组(Blood组,n=10);动态监测平均动脉血压(MAP),于休克前(T0)、休克末(T1)及复苏后1 h(T2)测定动脉血气指标,并记录24 h内大鼠的存活情况。结果:Ringer's组、HES组复苏后大鼠MAP仍较低,NCAHbs组复苏后MAP接近Blood组,并能有效维持;复苏后1 h,Blood组和NCAHbs组PCO2、PO2均得到改善,接近休克前基础水平;仅NCAHbs组及Blood组术后24 h生存率为100%。结论:新型淀粉纳米囊人工红细胞能够有效的扩容、携氧,明显改善重度失血性休克时机体和组织的氧供,是较为理想的人工氧载体。

Objective： A novel comprehensive strategy for the preparation of nanosized cationic amylose-encapsulated bovine hemoglobin（NCAHbs） as a nanosized oxygen carrier was developed.A lethal hemorrhagic shock model in rats was used to evaluate the efficacy of the NCAHbs.Methods： The healthy rats（weighted 200-250 g）were randomly divided into five groups： Control group（n=10）,Ringer＇s group（n=10）,HES group（hydroxyethyl starch,n=10）,NCAHbs group（n=10） and Blood group（n=10）.Hemorrhagic shock model was performed according to Wiggers＇ modified method.The mean arterial pressure（MAP） was monitored continuously.Arterial blood gas（ABG） were measured before shock（T0）,shock（T1） and 1 hour after resuscitation（T2）.The survival rates were recorded in 24 h after resuscitation.Results： Compared with Ringer＇s group and HES group after resuscitation,the MAP,PCO2and PO2of NCAHbs group almost reached the baseline and could effectively maintain;Only the survival rate of NCAHbs group and Blood group were 100% 24 h after resuscitation.Conclusions： The novel nanosized cationic amylose-encapsulated bovine hemoglobin（NCAHbs） could effectively against lethal hemorrhagic shock,because they were able to carry oxygen,significantly improve the tissue oxygen supply,which was an ideal artificial oxygen carrier.